Fig. 2. Isomer-specific regulation by CLA of insulin-stimulated glucose uptake and metabolism.

Cultures of SV cells containing newly differentiated human adipocytes were treated (TRT) continuously for 72 h with either a BSA vehicle or 30 μm cis-9, trans-11 CLA (9,11) or trans-10, cis-12 CLA (10,12). A, basal and insulin-stimulated uptakes of 4 nmol of 2-[³H]deoxyglucose were measured after a 90-min incubation in the absence (−) or presence (+) of 100 nm insulin; the basal control (BSA, –insulin) rate of uptake was ~100 pmol/(h·mg of protein). B, basal and insulin-stimulated de novo conversions of 2.2 nmol of [¹⁴C]glucose into [¹⁴C]lipid were measured after a 3-h incubation in the absence or presence of 100 nm insulin; the basal control rate was ~10 pmol/(h·mg of protein). C, basal and insulin-stimulated [¹⁴C]CO₂ production from 2.2 nmol of [¹⁴C]glucose were measured after a 3-h incubation in the absence or presence of 100 nm insulin; the basal control rate was ~11 pmol/(h·mg of protein). All data are expressed as a percentage of basal vehicle control (BSA, – insulin) rate. Means (± S.E.; n = 6) not sharing common superscript differ, p < 0.05.