Fig. 3. Isomer-specific regulation of FA uptake and metabolism by CLA.

Cultures of SV cells containing newly differentiated human adipocytes were treated (TRT) continuously for 72 h with either a BSA vehicle, 30 μm cis-9, trans-11 CLA (9,11) or trans-10, cis-12 CLA (10,12). A, [¹⁴C]oleic acid (12.5 nmol) uptake was measured after a 2-h incubation; the control rate of uptake was ~15 nmol/(h·mg of protein). B, [¹⁴C]oleic acid (12.5 nmol) incorporation into [¹⁴C]lipid was measured after a 2-h incubation; the control rate was ~ 12 nmol/(h·mg of protein). C, [¹⁴C]CO₂ production from [¹⁴C]oleic acid was measured after a 2-h incubation; the control rate was ~100 pmol/(h·mg of protein). Data are expressed as a percentage of vehicle control (BSA) rate. Means (± S.E.; n = 6) not sharing a common superscript differ, p < 0.05.