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. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: J Biol Chem. 2004 Apr 2;279(25):26735–26747. doi: 10.1074/jbc.M401766200

Fig. 4. Chronic trans-10, cis-12 CLA treatment alters gene expression.

Fig. 4

Cultures of SV cells containing newly differentiated human adipocytes were treated continuously for 8, 24, 72, or 216 h with either a BSA (♦) vehicle, or 30 μm cis-9, trans-11 CLA (•), or trans-10, cis-12 CLA (▴). After treatment, total RNA was harvested and used for first strand cDNA synthesis. Real time quantitative PCR analyses were performed to examine the expression of acyl-CoA-binding protein (ACBP), adiponectin, ap2, CAP, C/EBP-α, GLUT4, GPDH, leptin, lipoprotein lipase (LPL), perilipin, and PPAR-γ1 and PPAR-γ2. Results shown are representative of three separate experiments from independent human subjects.

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