Fig. 7. Trans-10, cis-12 CLA-induced alterations in adipocyte gene expression are blocked by pretreatment with the MEK inhibitor U0126.

Cultures of SV cells containing newly differentiated human adipocytes were serum starved for 24 h and pretreated with or without 10 μm U0126 for 1 h. Subsequently, cultures were treated with either a BSA vehicle or 30 μm trans-10, cis-12 CLA for an additional 24 h. After 24 h of treatment, total RNA was harvested and used for first strand cDNA synthesis. Real time quantitative PCR analyses were performed to examine the expression of adiponectin, aP2, C/EBP-α, GLUT4, GPDH, leptin, lipoprotein lipase (LPL), perilipin, and PPAR-γ1 and PPAR-γ2. Results shown are representative of four separate experiments from independent human subjects.