Fig. 8. Trans-10, cis-12 CLA-induced alterations in glucose and FA uptake are blocked by pretreatment with U0126 and PTX.

Cultures of SV cells containing newly differentiated human adipocytes were serum starved for 24 h and pretreated with or without 10 μm U0126 or 100 ng/ml PTX for 1 h. Subsequently, cultures were treated with either a BSA vehicle or 30 μm trans-10, cis-12 CLA complexed to BSA for an additional 24 h. A, insulin-stimulated uptake of 4 nmol of 2-[³H]deoxyglucose was measured after a 90-min incubation in the absence or presence of 100 nm insulin; the control rate of uptake was ~130 pmol/(h·mg of protein). B, [¹⁴C]oleic acid (12.5 nmol) uptake was measured after a 2-h incubation; the control rate of uptake was ~11 nmol/(h·mg of protein). Data are expressed as a percentage of vehicle control (BSA) rate. Means (± S.E.; n = 6) not sharing common superscripts differ, p < 0.05.