TABLE 2.
Heparin-, heparan sulfate-, and chondroitin sulfate-degrading activities in native and transconjugant F. heparinum strainsa
| Growth medium | Activity (mIU ml−1 OD600−1)b
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Hc
|
HS | CSA
|
CSC
|
DS
|
|||||
| MHd | MA | MH | MH | MA | MH | MA | MH | MA | |
| Native | 158 ± 12 | 102 ± 10 | 107 ± 9 | 15 ± 1 | 99 ± 9 | 13 ± 1 | 72 ± 9 | 30 ± 2 | 64 ± 5 |
| FIBX1 | 126 ± 8 | 100 ± 4 | 93 ± 7 | 14 ± 2 | 115 ± 4 | 12 ± 2 | 82 ± 5 | 27 ± 2 | 96 ± 14 |
| FIBX3 | 144 ± 31 | NDe | 174 ± 24 | ND | ND | ND | ND | ND | ND |
| FIBX4 | 179 ± 13 | ND | 844 ± 37 | ND | ND | ND | ND | ND | ND |
| FIBX5 | 775 ± 89 | ND | 63 ± 4 | ND | ND | ND | ND | ND | ND |
| FIBX6 | 119 ± 19 | 110 ± 7 | ND | 2,376 ± 135 | 4,057 ± 254 | 1,510 ± 87 | 2,620 ± 399 | 59 ± 4 | 126 ± 9 |
| FIBX7 | 143 ± 12 | 104 ± 10 | ND | 23 ± 3 | 111 ± 4 | 14 ± 3 | 72 ± 2 | 927 ± 134 | 958 ± 97 |
a
Values are the average of three separate experiments ± the standard error.
b
One international unit of GAG-degrading activity is defined as the amount of enzyme that will produce 1 μmol of product per min at 30°C at pH 8.
c
Substrates for GAG lyases' activity assay: H, heparin; HS, heparan sulfate; CSA, chondroitin sulfate A; CSC, chondroitin sulfate C; and DS, dermatan sulfate.
d
Media used in this study. MH, FM supplemented with 1% heparin; and MA, FM supplemented with 1% chondroitin sulfate A.
e
ND, not determined.