TABLE 3.

Heparin- and chondroitin sulfate A-degrading activities in native F. heparinum and FIBX6 when grown in various media^a

Growth mediume	Activity (mIU ml−1 OD600−1)b
	Native F. heparinum		FIBX6
	Hc	CSAd	H	CSA
1	158 ± 12	15 ± 1	119 ± 19	2,376 ± 135
2	102 ± 10	99 ± 10	110 ± 7	4,057 ± 254
3	<5	11 ± 2	<5	16 ± 2
4	0 ± 6	6 ± 2	37 ± 11	814 ± 54
5	<5	<5	<5	17 ± 2
6	<5	<5	<5	11 ± 2
7	31 ± 3	5 ± 1	27 ± 2	187 ± 21
8	117 ± 8	9 ± 2	112 ± 8	748 ± 26

^aValues are the average of three separate experiments ± the standard error.

^bOne international unit of heparin- or chondroitin sulfate A- degrading activity is defined as the amount of enzyme that will produce 1 μmol of product per min at 30°C at pH 8.

^cSubstrate heparin.

^dSubstrate chondroitin sulfate A.

^eMedia: 1, FM supplemented with 1% heparin (data taken from Table 2); 2, FM supplemented with 1% chondroitin sulfate A (data taken from Table 2); 3, FM supplemented with 0.4% glucose; 4, FM supplemented with 0.4% glucose and 1% heparin; 5, LB medium; 6, LB medium supplemented with 0.4% glucose; 7, LB medium supplement with 0.4% glucose and 1% heparin; and 8, LB medium supplement with 1% heparin.