FIG. 3.
Binding of the chimeric flagella to the human Intestine 407 cells. The bound flagella were detected by indirect immunofluorescence using anti-FliCH7 immunoglobulin G and fluorochrome-conjugated secondary antibodies. (A) Binding of the SlpA 96-245/FliC chimeric flagella. (B) The corresponding field under light microscopy. (C) Binding of SlpA 239-447/FliC. (E) Binding of the ΔFliC flagella lacking an insert. (D and F) Light microscopic fields corresponding to panels C and E, respectively. Arrows indicate cell contours. Bar, 20 μm.