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. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: Pharmacogenet Genomics. 2005 Apr;15(4):245–256. doi: 10.1097/01213011-200504000-00008

Fig. 4.

Fig. 4

Identification of the g.10,951delG SNP (encodes p.V113fs) by SSCP detection (b) of products produced from standard 2-primer (hF2–83 and hF2–49r) PCR amplification (a). (a) Control reactions to confirm 120-bp PCR product amplification. Lane 1: MspI cut pBR322. Lanes 2–7 control reactions: 2, minus template; 3, synthetic heterozygote composed of reference plasmid + g.10,951delG plasmid DNA; 4, reference plasmid DNA; 5, g.10,951delG plasmid DNA; 6, genomic DNA from a reference individual; 7, genomic DNA with added g.10,951delG plasmid DNA. Lanes 8–11 are products from genomic DNA from individual Hispanic-American samples. (b) SSCP separation of control products from: 1, synthetic heterozygote composed of reference plasmid + g.10,951delG plasmid DNA; 2, reference plasmid DNA; 3, g.10,951delG plasmid DNA. Lanes 4–13 show SSCP separation of products from individual Hispanic-Americans.