Table 2.

Primers used for allele-specific and SSCP genotyping

Primera	Description	Sequence (5′ to 3′)b
Exon 3: g.7,700_7,702dupGAC polymorphism (p.71Ddup)
hF2–44	wild-type forward	AGA AAT GTC CTG TTT CAG TGc CT
hF2–45	71Ddup forward	gcc ccg ggg gCA Aga AAA TGT CCT GTT TCA GTc ACG
hF2–46r	common reverse	TTA TGC AGG AAG TTT GG
Exon 4: g.10,951delG polymorphism (p.V113fs)
hF2–83	common forward	TTC AAG ACA ACT GTC CTT A
hF2–49r	common reverse	AAC CAT AAC TGC GTC AAA G
Exon 5: g.13,732C > T polymorphism (p.S195L)
hF2–50	wild-type forward	ggg ggg ggg ggc CCT caT GAT TGG AAT GGG AAC cTC
hF2–79	S195L forward	CTG GTG ATT GGt ATG GGA AAg TT
hF2–52r	common reverse	TGA GCA GCA TTC TTA CTC AG
Exon 8: g.22,060T > G polymorphism (p.N413 K)
hF2–59	common forward	TTG TGT AGC CTG CCC TCA GA
hF2–60r	wild-type reverse	ccc ccc ccc cgt GGT CAA TTC TTT TgT CA
hF2–61r	N413 K reverse	CAc GTC AAT TCT TTT TaC C
Exon 9: g.23,238C > T polymorphism (p.Q472X)
hF2–42	FMO2*1 forward	CGG ACC CTG CAA CTC CTt TC
hF2–20	FMO2*2 forward	caa gct tat aTA TTT CGG ACC CTG CAA gTC CTA TTc
hF2–18r	Common reverse	caa agc TTA TGC TGA CTA GGc

^aLower case ‘r’ indicates a reverse primer, while absence of an ‘r’ indicates a forward primer.

^bLower case indicates intentional sequence mismatches; bold/underlining indicates the polymorphism discriminating nucleotide.

^cThese primers have been described previously [16].