Growth curves of M. smegmatis strains at 30 and 42°C. The growth of the control strain, M. smegmatis mc2155, containing plasmid pYM201 at 30°C (▴) and at 42°C (▵), and the growth of the rmlD knockout strain, M. smegmatis YM202, containing plasmid pYM201 at 30°C (•) and at 42°C (○), are illustrated.