FIG. 3.

Deletion analysis of PucR-induced promoters. The sequences upstream of the −35 sequence of the indicated gene-lacZ fusions, including the PucR box, are shown. The downstream sequence fusion points are +97 for pucH′-lacZ, +74 for pucI′-lacZ, +137 for pucJ′-lacZ, +94 for pucF′-lacZ, and +78 for gde′-lacZ. In the case of pucJ, the upstream TnrA/GlnR box (Fig. 2B) is also indicated. Cells containing the various lacZ fusions were grown under nitrogen-limiting conditions (glutamate) either with or without allantoin. nt, nucleotides. Boldface indicates a match to the consensus sequence.