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. 2002 Jun;184(12):3232–3241. doi: 10.1128/JB.184.12.3232-3241.2002

FIG. 5.

FIG. 5.

Site-directed mutational analysis of the proposed PucR box in the pucH and pucI regulatory regions. The sequences upstream of the −35 region of the pucH′-lacZ and pucI′-lacZ fusions are listed. The PucR box is indicated (boldface indicates a match to the consensus sequence), and the positions of the upstream fusion points are shown. The downstream sequence fusion points are +97 for pucH′-lacZ and +74 for pucI′-lacZ. Cells containing the various lacZ fusions were grown under nitrogen-limiting conditions (glutamate) either with or without allantoin. wt, wild type.