TABLE 2.
β-Galactosidase produced from transcriptional fusionsa
| Strain | Relevant genotype | β-Galactosidase activity (U/mg of protein)
|
||
|---|---|---|---|---|
| Glutamate plus NH4+ | Glutamate | Glutamate plus allantoin | ||
| HH422 | amyE::pucA′-lacZ | 1 | 335 | 12 |
| CJ13 | amyE::pucA′-lacZ pucR::pBOER | 1 | 145 | 117 |
| LB032 | amyE::pucF′-lacZ | 1 | 37 | 1,818 |
| CJ12 | amyE::pucF′-lacZ pucR::pBOER | 1 | 2 | 19 |
| CO7 | amyE::pucH′-lacZ | 2 | 17 | 354 |
| CJ10 | amyE::pucH′-lacZ pucR::pBOER | 2 | 2 | 3 |
| HØR19 | amyE::pucI′-lacZ | 1 | 23 | 400 |
| HØR29 | amyE::pucI′-lacZ pucR::pBOER | 1 | 10 | 60 |
| LB035 | amyE::pucJ′-lacZ | 2 | 1,002 | 2,336 |
| CJ14 | amyE::pucJ′-lacZ pucR::pBOER | 1 | 3 | 8 |
| CO3 | amyE::pucR′-lacZ | 1 | 27 | 6 |
| LB175 | amyE::pucR′-lacZ pucR::pBOER | 1 | 508 | 420 |
| LB031 | amyE::gde′-lacZ | 4 | 12 | 160 |
| ED441 | amyE::gde′-lacZ pucR::pBOER | 5 | 5 | 3 |
a
Fusions were between lacZ and the regulatory region upstream of pucA, pucF, pucH, pucI, pucJ, pucR, or gde integrated at the amyE locus of the wild-type B. subtilis strain and a pucR derivative grown under different nitrogen conditions. Cells were grown in glucose minimal medium supplemented with the indicated nitrogen source.