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. Author manuscript; available in PMC: 2006 Aug 1.
Published in final edited form as: Pigment Cell Res. 2005 Aug;18(4):265–277. doi: 10.1111/j.1600-0749.2005.00234.x

Figure 6.

Figure 6

Synergistic transcriptional activities are enhanced by sumoylation site mutations of MITF. (A) Schematic illustration of GAL4-responsive reporter genes used in this study. (B) Expression plasmids for GAL4 DNA binding domain fused with the wild type and 2KR mutant of MITF were transfected into NIH3T3 cells with a luciferase reporter gene containing one (1X GAL), three (3X GAL) or five (5X GAL) GAL4 binding sites upstream of the TATA box and phRL. (C) Ratio of mutant over WT activity measured in Figure 5B. (D) Schematic representation of promoter fragment of the human Dct gene. Effects of the 2KR mutation on the synergistic transcription between MITF and SOX10. Expression plasmids for wild type or 2KR mutant MITF, wild type or SOX10, a luciferase reporter gene containing the DCT promoter, and phRL-TK were cotransfected into NIH3T3 cells. Luciferase activity was measured as described for Figure 5.