A. The invasive potential of mock and cells transfected with the indicated shRNA plasmids were examined by matrigel invasion assay (visual field representative of one experiment). Invasion assays performed as described above (see Fig. 1C).
B. Representative number of invading cells through the matrigel was counted under microscope in three random fields at 200X. Each bar represents the mean ± SD of three fields counted. Significant difference from controls (i.e., mock or scrambled vector-transfected cells) is indicated by asterisks * (P <0.05).
(Results are representative of three separate experiments.)