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. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: J Bone Joint Surg Am. 2003;85-A Suppl 3:19–28. doi: 10.2106/00004623-200300003-00005

Fig. 1.

Fig. 1

Alkaline phosphatase (AP) activity in day-6 first-passage human marrow-derived stromal cell cultures. First-passage human marrow-derived stromal cell cultures were treated and were harvested for analysis of alkaline phosphatase activity as described in Materials and Methods. A: First-passage cultures derived from untreated primaries did not show any response to BMP-2 treatment. Alkaline phosphatase activity was significantly (*) increased by dexamethasone (Dex). B: In first-passage cultures derived from dexamethasone-treated primaries, BMP-2 significantly increased alkaline phosphatase activity. Dexamethasone pretreatment also enhanced the dexamethasone stimulation of alkaline phosphatase seen in the first passage. C: The influence of dexamethasone pretreatment on BMP-2 induction of alkaline phosphatase in the first passage was greater in cultures of cells derived from male patients than it was in cultures of cells from female patients.