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. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: J Bone Joint Surg Am. 2003;85-A Suppl 3:19–28. doi: 10.2106/00004623-200300003-00005

Fig. 3.

Fig. 3

Expression of BMP-inducible genes in a representative sample of cultured first-passage human marrow-derived stromal cells. cDNA was prepared from mRNA of day-6 first-passage cultures and analyzed by real-time reverse transcriptase-polymerase chain reactions. SYBR Green fluorescence indicates formation of polymerase chain reaction product. The cycle number at which product fluorescence crosses the fluorescence threshold is the crossing point; larger amounts of initial mRNA result in detectable fluorescence at a lower crossing point. Both noggin and BMP-2 mRNA were increased in BMP-treated cultures. Dexamethasone (Dex) treatment increased noggin mRNA but decreased BMP-2 mRNA.