Fig. 4.
Immunoblot analysis for noggin protein in human marrow-derived stromal cells. Whole cell protein lysates from day-6 cultures were evaluated as described in Materials and Methods with use of a rat anti-human noggin antibody. Two representative samples from non-pretreated first-passage cultures are shown. The results mirror the reverse transcriptase-polymerase chain reaction results, demonstrating increased noggin protein associated with either BMP-2 or dexamethasone (Dex) treatment and the additive effect of combined treatment.