FIG. 5.

Temporal effects of glucose on biofilm formation at 24 or 48 h of growth. Glucose (0.2% [wt/vol], final concentration) was added at the indicated times after inoculation of MG1655 or its csrA mutant, TRMG1655, into CFA or LB medium. Crystal violet staining was measured at A₆₃₀ at either 24 or 48 h of growth, and results were calculated as percentages of values for the control cultures that lacked glucose. Each point is the average and standard error of three separate experiments. ∗ and ∗∗, significant decrease or increase (P < 0.0001), respectively, in biofilm formation relative to controls lacking glucose.