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. Author manuscript; available in PMC: 2006 Jun 5.
Published in final edited form as: FEBS Lett. 2005 Nov 14;579(29):6681–6687. doi: 10.1016/j.febslet.2005.10.064

Fig. 2.

Fig. 2

Gemcitabine induced apoptosis via caspase activation in H1299 cells. (A) Flow cytometry analysis of H1299 and H1299/GR cells treated with 100 nM gemcitabine for the indicated time periods. Cells were harvested, fixed, and stained with PI. Histograms represent DNA contents. The numbers represent the percentages of sub-G1 cells. (B) H1299 and H1299/GR cells were treated with 100 nM gemcitabine for the indicated time periods, and proteins in whole-cell lysates were analyzed by immunoblotting with anti-caspase-3, caspase-8, and caspase-9 antibodies. The arrowheads represent cleavage proteins. β-actin was used as a loading control.