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. 2026 Jul 27;33(8):450. doi: 10.3390/curroncol33080450
Method %MAF Err. Rate TAT Cost-per-Sample
Sanger sequencing 10–20% 10−1 1 day low
Quantitative PCR (qPCR) 5–10% 10−3–10−2 3–5 h very low
NGS (amplicon based) 1% 10−3–10−2 2–3 days medium
NGS (hybrid capture based) 0.1% 10−3–10−2 3–5 days high
Allele specific PCR (ARMS) 0.1% 10−3 3–5 h low
NGS (with UMI) 0.01% 10−5 4–7 days very high
Droplet-digital PCR 0.01% 10−4 6–8 h low