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. 2002 Jun;184(12):3296–3304. doi: 10.1128/JB.184.12.3296-3304.2002

FIG. 2.

FIG. 2.

Analysis of the inhA2 promoter region. (A) Sequence of the inhA2 promoter region. The putative −10 and −35 boxes of the inhA2 promoter are boxed. The potential ribosome binding site and the start codon (ATG) are underlined. The potential reverse Spo0A box is indicated in bold. The broken arrow indicates the transcriptional start site. (B) Mapping of the inhA2 transcriptional start site by primer extension. Total RNA was isolated from B. thuringiensis strain 407 Cry− 1 h (t−1) before and 0 (t0) and 2 h (t2) after the beginning of the stationary phase. RNA was subjected to primer extension using the oligonucleotide Bext1. Lanes A, C, G, and T show the sequence of the promoter region of the inhA2 gene.