Fig. 1.

Ror1 and Ror2 suppression by antisense oligonucleotide treatment in cultured hippocampal neurons. 2-day cultured control (A,C,E,G), Ror1 antisense- (B,D) and Ror2 antisense- (F,H) treated hippocampal neurons were double stained with tubulin (A,B,E,F) and Ror1 (C,D) or Ror2 (G,H) antibodies. Ror1 and Ror2 were readily detectable in the soma, the neurites and the growth cones of control neurons (C,G). On the other hand, very little Ror immunoreactivity was found in many of the neurons treated with Ror1 or Ror2 oligonucleotides (arrows in D,H). Tubulin expression was not affected by Ror1 and Ror2 antisense oligonucleotide treatment (B,F). ax, axon; mp, minor process. Bar, 20 μm.