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. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: J Cell Sci. 2005 Jan 15;118(Pt 2):433–446. doi: 10.1242/jcs.01622

Fig. 3.

Fig. 3

Reduction of Ror1 and Ror2 expression by RNAi in hippocampal cultures. Western blot analysis of whole cell extracts prepared from 2-day in vitro untreated hippocampal cultures (A and B, lane 1), cultures that had been transfected with Ror1 (A, lane 2) or Ror2 (B, lane 2) siRNA duplexes and cultures that had been transfected with the corresponding scrambled oligomers (A and B, lane 3). The proteins were separated by SDS-PAGE and immunoblots were reacted with Ror1 (A) and Ror2 (B) antibodies. Densitometries of the immunoreactive bands are shown under the blot. Values were normalized using tubulin as an internal control. The results correspond to the percentage of Ror1 and Ror2 protein present in the RNAi-transfected cultures as compared to the levels detected in the untreated controls (100%). *P<0.01 compared to levels in untreated and scrambled siRNA-treated cultures.