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. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: J Cell Sci. 2005 Jan 15;118(Pt 2):433–446. doi: 10.1242/jcs.01622

Fig. 8.

Fig. 8

Ror suppression is accompanied by changes in the levels of the microtubule-associated proteins MAP1B and MAP2. Western blot analysis of whole cell extracts prepared from 2-day in vitro untreated hippocampal cultures (A and B, lane 1), cultures that had been transfected with Ror1 (A, lane 2) or Ror2 (B, lane 2) siRNA duplexes and cultures that had been transfected with the corresponding scrambled siRNAs (A and B, lane 3). Densitometries of the immunoreactive bands obtained by western blotting with MAP1B, MAP2 and tau antibodies are shown below the blots. The results correspond to the percent of MAP1B, MAP2 and tau protein levels present in the siRNA-transfected cultures when considering the levels in the control samples as 100%. Note the significant decrease of MAP1B and MAP2, but not tau, levels in the siRNA Ror1 and Ror2-treated cultures when compared to levels in control cells. *P<0.01 when compared to levels in untreated and scrambled siRNA-treated cultures.