Fig. 5.
Phosphorylation of Ras downstream effectors in CCSP/Ki-rasG12C mice ± treatment with 500 μg/ml of DOX for 3, 6 or 9 months. Lung tissue was removed, embedded in paraffin, and 5 μm sections were cut for immunohistochemistry with phospho-specific antibodies. (A) Anti-phospho-ERK, 400×; (B) anti-phospho-p90 RSK, 400×; (C) anti-phospho-pS6, 400×; (D) anti-phospho-p38, 400; (E) anti-phospho-MAPKAPK-2, 400; (F) anti-phospho-JNK, 400×; (G) anti-phospho-Akt, 400×.