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. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: J Med Chem. 2005 Oct 6;48(20):6236–6249. doi: 10.1021/jm0503547

Figure 5.

Figure 5

Cell viabilities of Caco-2 monolayers and Jurkat cells treated with synthetic peptides. The MTT assay was applied to examine the cytotoxicity of peptides to the Caco-2 and Jurkat cells during the lymphocyte–epithelial adhesion assay. The highest concentration of peptide used in the adhesion assay was added to the cells and incubated for the time of exposure of Caco-2 and Jurkat cells during the adherence assay. The cell viabilities were validated by incubating the cells with 5 mg/mL MTT for 3 h at 37 °C. The MTT-labeled cells were then solubilized in DMSO before the color of the reaction product was quantified using a plate reader. All samples were assayed in four independent assays, and the mean for each experiment was calculated.