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. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: Nat Med. 2005 Mar 6;11(4):440–445. doi: 10.1038/nm1204

Fig. 1. Wild type GFP-lamin A is insufficient for phenotypic rescue of HGPS cells.

Fig. 1

(a–c) Immunofluorescence microscopy on primary dermal fibroblasts from a healthy control individual (AG08469; population doublings: 25–30) (a) and a HGPS patient (AG01972; population doublings: 25–30), untreated (b) or transfected with wild type lamin A and GFP as a transfection marker (c). Cells were stained with DAPI (blue) and antibodies (red) against the indicated proteins. Four cells (indicated by arrowheads) with normal staining respectively for lamin B, LAP2, HP1α and Tri-Me-K9 are shown in panel b to directly compare the cellular level of the proteins in unaffected and affected cells. The percentage of cells showing aberrant phenotype is indicated. Scale bar: 10 μm. (d) FRAP analysis of wild type GFP-lamin A in living control and HGPS cells. Nuclei were imaged before and during recovery after the bleach pulse. Scale bar: 5 μm. (e) Kinetics of recovery of the fluorescence signal in the whole bleached area. The statistical significance of the difference between the two recovery curves is indicated. (f) Immunofluorescence microscopy on primary dermal fibroblasts from a healthy control individual (AG08469; population doublings: 25–30) transfected with GFP-Δ50 lamin A. Cells were stained with DAPI (blue) and antibodies (red) against lamin A/C, lamin B, LAP2 proteins. The green fluorescent signal from GFP-Δ50 lamin A is overlaid with the DAPI signal. Scale bar: 10 μm.