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. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: J Biol Chem. 2005 Jun 27;280(35):31019–31026. doi: 10.1074/jbc.M503690200

Fig. 2. SDS-PAGE/Western analysis of semisynthetic Gα.

Fig. 2

Ligated product was observed after 24 h. Lane 1, purified Gαi1ΔCT thioester (negative control); lane 2, purified Gαi1 (N346C). Time points were taken at 24 (lanes 3 and 4), 48 (lanes 5 and 6), and 72 h (lanes 7 and 8) and treated with (+) or without (−) 15 mm dithiothreitol before separation on 10% SDS-polyacrylamide gels. Products were transferred to membranes and blotted with anti-carboxyl-terminal Gαi1 antibody.