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. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: J Biol Chem. 2005 Jun 27;280(35):31019–31026. doi: 10.1074/jbc.M503690200

Fig. 3. C5a receptor activation of ligated G proteins.

Fig. 3

C5a receptors (1–5 nm) in urea-stripped membranes were incubated with C5a ligand (100 nm) and Gα proteins (1 μm) and Gβγ (0.5 μm) and GTPγS (3 μm) in 20-μl reactions for 10 min at 30 °C and then filtered and counted. Data shown are the average ± S.E. of six experiments.