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. 2002 Jun;184(12):3260–3267. doi: 10.1128/JB.184.12.3260-3267.2002

FIG. 5.

FIG. 5.

In vivo copurification of the H. volcanii SRP components. (a) Copurification with Hv54h•6xHis. The cytoplasmic fractions of strains WRHv-6c (Hv54h•6xHis) and WRHv-NP15 (control) were purified with Ni-NTA, and the cytoplasmic (cyto) and elution (elut) fractions were analyzed by Western blotting with antibodies against Hv54h and 6xHis (detecting Hv54h•6xHis), as well as by Northern blotting with a DNA probe for Hv7Sh. (b) Copurification with Hv19h•6xHis. The cytoplasmic fractions of WRHv-9a (Hv19h•6xHis) and WRHv-NP15 (control, not shown) were purified with Ni-NTA, and the elution fractions (E1 and E2) were analyzed by Western blotting with antibodies against 6xHis tag (detecting Hv19 h•6xHis) and Hv54h, as well as by Northern blotting with a DNA probe for Hv7Sh. The lower band observed in the anti-pentaHis Western blot is likely a degradation product of Hv19h•6xHis.