TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source and/or reference |
|---|---|---|
| Strain | ||
| WFD11 | H. volcanii | 5 |
| MP12 | E. coli MC4100 | 29 |
| WAM113 | E. coli ffh conditional mutant | 27 |
| WR4 | MP12 containing pWR-4 | This study |
| WR6c | MP12 containing pWR-6c | This study |
| WAM-ffh | WAM113 containing pRB11-ffh | This study |
| WAM-hv54h | WAM113 containing pRB11-hv54h | This study |
| WRHv-NP15 | WFD11 containing pNP15 | This study |
| WRHv-6c | WFD11 containing pWR-6c | This study |
| WRHv-6c/54KO | WRHv-6c with mevR insertion in chromosomal hv54h | This study |
| WRHv-9a | WFD11 containing pWR-9a | This study |
| Plasmid/cosmid | ||
| A199chr, 347chr | Kanr; cosmid vectors (Lorist M) containing H. volcanii chromosomal MluI fragments | 6 |
| pNP15 | Ampr Nbr; H. volcanii shuttle vector | 26 |
| pMDS99 | Ampr; plasmid containing hhmevR gene | 34 |
| pBAD18 | Ampr; arabinose-inducible expression plasmid | 14 |
| pMAL-c2 | Ampr; IPTG-inducible expression plasmid encoding N-terminal maltose binding protein | 37 |
| p105-SacI | Ampr; pBAD18 containing SacI fragment of cosmid A199chr | This study |
| p64-AluI | Ampr; pBAD18 containing AluI fragment of cosmid 347chr | This study |
| pWR-2 | Ampr; pBAD18 containing hv54h ORF + 66 bp | This study |
| pWR2-hhmevR | pWR-2 with hv54h interrupted by hhmevR | This study |
| pWR-4 | Ampr; pMAL-c2 containing hv54h ORF | This study |
| pWR-6c | pNP15 containing hv54h-6xhis | This study |
| pWR-9a | pNP15 containing hv19h-6xhis | This study |
| pRB11-ffh | pRB11 containing ffh | 33 |
| pRB11-hv54h | pRB11 containing hv54h | This study |