FIG. 4.
Alignment of chloromethane specific promoter regions in M. chloromethanicum CM4. The putative −35 and −10 regions are indicated in boldface, and experimentally determined transcription initiation sites are underlined. A likely consensus promoter is shown. Promoter regions of previously identified Methylobacterium genes involved in one-carbon metabolism are also shown for comparison. PdcmA, dichloromethane dehalogenase promoter of M. dichloromethanicum (14); PmxaF(AM1), methanol dehydrogenase promoter of M. extorquens AM1 (2); PmxaF(XX), methanol dehydrogenase promoter of Methylobacterium organophilum XX (16). The E. coli σ70 consensus promoter sequence (43) is also given for comparison.