Table 1.
Binding of human mesothelin peptides and analog peptides to HLA-A2 molecules
| Experiment 1: Analysis of native human mesothelin peptide to HLA-A2 molecules | |||
|---|---|---|---|
| Peptide | Amino acid position in mesothelin | Sequence | T2 bindinga |
| P21 | 21-29 | FLLFSLGWV | 241 (1.5) |
| P547 | 547-556 | KLLGPHVEGL | 407 (2.6) |
| MUC-1 (positive control) | NA | ALWGQDVTSV | 422 (2.7) |
| CAP-7 (negative control) | NA | HLFGYSWYK | 152 |
| Experiment 2: Analysis of analog peptides | |||
| Mesothelin agonist peptides derived from P547 | Designation | Peptide sequence | T2 bindinga |
| P547 (native) | P547 | KLLGPHVEGL | 226 (1.5) |
| 554L | P547-1 | KLLGPHVLGL | 180 (1.2) |
| 554L/556V | P547-2 | KLLGPHVLGV | 289 (2.0) |
| 548M/554L/556V | P547-3 | KMLGPHVLGV | 368 (2.6) |
| 548I/554L/556V | P547-4 | KILGPHVLGV | 342 (2.3) |
| 548M/554L | P547-5 | KMLGPHVLGL | 132 (0.9) |
| 548I/554L | P547-6 | KILGPHVLGL | 185 (1.3) |
| 547Y/554L/556V | P547-7 | YLLGPHVLGV | 234 (1.6) |
| 547Y/554L | P547-8 | YLLGPHVLGL | 161 (1.1) |
| MUC-1 peptide (positive control) | ALWGQDVTSV | 390 (2.7) | |
| CAP-7 (negative control) | HLFGYSWYK | 144 | |
a
Results are expressed in mean fluorescence intensity (MFI). MUC-1 peptide is an HLA-A2 binding peptide and CAP-7 is an HLA-A3 binding CEA peptide.
NA, not applicable.
Peptides were used at a concentration of 25 μg/ml. Values in parentheses are fold increases as compared with the negative control. Amino acid sequences of the parental P547 peptide (amino acid position 547-556 of mesothelin) and analog peptides. Amino acids are shown by the single-letter code. Substitution amino acids are indicated in bold italic and underlined.