Table 4.

Production of lymphotactin by T-cell lines generated from a pancreatic cancer patient (patient 55) stimulated with P547 and the agonist peptides

	Production of lymphotactin (pg/ml)
T-cell line	Corresponding peptide	CAP1-6D
T-55-547	<31.2	<31.2
T-55-P547-2	2,843	<31.2
T-55-P547-3	<31.2	<31.2
T-55-P547-4	73.5	<31.2

Cells from four mesothelin-specific T-cell lines established from a pancreatic cancer patient (patient 55) were used as effector cells at in vitro stimulation (IVS-3). These T-cell lines were established by stimulation with P547-pulsed autologous DCs (T-55-P547), P547-2–pulsed autologous DCs (T-55-P547-2), P547-3–pulsed autologous DCs (T-55-P547-3) or P-547-4–pulsed autologous DCs (T-55-P547-4). For lymphotactin production, T-cell lines were stimulated with the corresponding peptide at a concentration of 25 μg/ml and an effector-to-APC ratio of 10:1. CAP1-6D peptide was used as a negative control. Twenty-four-hour culture supernatants were collected and screened for the secretion of lymphotactin.