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. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: Mol Ther. 2005 Oct 6;12(6):1217–1225. doi: 10.1016/j.ymthe.2005.08.018

FIG. 4.

FIG. 4

Western blot analysis of Rep proteins expressed in Sf9 cells by BacRep, BacRep52, or BacRep78 baculovirus helpers individually or upon co-infection with other baculovirus helpers (m.o.i. of 5 each). Lane 1, positive control (a lysate from 293 cells transfected with pIM45 [21]; lanes 2 through 6 contain lysates from Sf9 cells infected with, lane 2, BacRep; lane 3, BacRep52; lane 4, BacRep78; lane 5, BacRep78 + BacRep52; lane 6, BacRep78 + BacRep52 + BacVP + BacGFP (the latter vector also contains a strong baculovirus p10 promoter driving the GFP gene inside the transgene cassette [15]).