TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | endA1 hsdR17 (rK− mK+) supE44 thi1 recA1 gyrA relA1 Δ(lacZYA-argF)U169 φ80ΔlacZM15 | 11 |
| ZI418 | araD139 ΔlacU169 rpsL 150 relA1 flbbB5301 deoC1 ptsF25 rbsR thi aroB fecB::Mud1 (Ap, lac) | 43 |
| MO704 | fecB::Mud1 (Ap, lac) fecl::KanraroB araD139 ΔlacU169 rpsL 150 relA1 flbB5301 deoC1 ptsF25 rbsR thi | 43 |
| SU202 | lexA71::Tn5 sulA211 sulA(op408/op+)::lacZ Δ(lacIPOZYA) 169/F′ lacIqlacZΔM15::Tn9 | 5 |
| P. aeruginosa | ||
| PAO1 | Wild type | Lab stock |
| Plasmids | ||
| pHSG576 | pSC101 derivative, Cmr | 40 |
| pSV66 | pHSG576 fecI fecR fecA | 12 |
| pMMO202 | pHSG576 NdeI/BglI (cleavage sites removed) | 38 |
| pMMO203 | pMMO202 fecI fecR | 38 |
| pSM39 | pMMO202 fecIΔ9-32 fecR | This study |
| pSM40 | pMMO202 fecIΔ32-55 fecR | This study |
| pSM41 | pMMO202 fecIΔ52-67 fecR | This study |
| pSM42 | pMMO202 fecIΔ65-83 fecR | This study |
| pSM43 | pMMO202 fecIΔ79-114 fecR | This study |
| pSM44 | pMMO202 fecIΔ114-134 fecR | This study |
| pSM53 | pMMO202 fecI(T1570C)(L113P) fecR | This study |
| pSM54 | pMMO202 fecI(T1642C)(L137P) fecR | This study |
| pSM55 | pMMO202 fecI(T1642G)(L137R) fecR | This study |
| pSM56 | pMMO202 fecI(T1669C/C1670G)(L146P) fecR | This study |
| pSM57 | pMMO202 fecI(T1669G/C1670G)(L146R) fecR | This study |
| pSM59 | pMMO202 fecI(T1729C)(L166P) fecR | This study |
| pSM60 | pMMO202 fecI(C1728A/T1729G)(L166R) fecR | This study |
| pMS604 | oriColE1 TetrlexA1-87-fos zipper | 5 |
| pSM173 | pMS604 lexA1-87-fecI | 8 |
| pSM20 | pMS604 lexA1-87-fecI Δ9-32 | This study |
| pSM21 | pMS604 lexA1-87-fecI Δ32-55 | This study |
| pSM22 | pMS604 lexA1-87-fecI Δ52-67 | This study |
| pSM23 | pMS604 lexA1-87-fecI Δ65-83 | This study |
| pSM24 | pMS604 lexA1-87-fecI Δ79-114 | This study |
| pSM25 | pMS604 lexA1-87-fecI Δ114-134 | This study |
| pSM26 | pMS604 lexA1-87-fecI Δ133-173 | This study |
| pSM76 | pMS604 lexA1-87-fecI64-173 | This study |
| pSM45 | pMS604 lexA1-87-fecI64-173(T1570C)(L113P) | This study |
| pSM49 | pMS604 lexA1-87-fecI64-173(T1642C)(L137P) | This study |
| pSM46 | pMS604 lexA1-87-fecI64-173 (T1669C)(L146P) | This study |
| pSM52 | pMS604 lexA1-87-fecI64-173 (A1613G/T1669G/T1702C)(E127E/L146R/V161A) | This study |
| pSM47 | pMS604 lexA1-87-fecI64-173 (T1729C)(L166P) | This study |
| pSM51 | pMS604 lexA1-87-fecI64-173 (T1729C/T1749G)(L166P/L173V) | This study |
| pSM61 | pMS604 lexA1-87-fecI109-173 | This study |
| pSM74 | pSM604 lexA1-87-fecI Δ132-148 | This study |
| pSM75 | pSM604 lexA1-87-fecI1-153 | This study |
| pDP804 | ori p15A AmprlexA1-87408-jun zipper | 5 |
| pSM85 | pDP804 lexA1-87408-fecR1-85 | 8 |
| pSM84 | pDP804 lexA1-87408-fecR | This study |
| pSM65 | pMS604 lexA1-87-pa24681-172 | This study |
| pSM66 | pMS604 lexA1-87-pa2468110-172 | This study |
| pSM68 | pMS604 lexA1-87-pa38991-170 | This study |
| pSM69 | pMS604 lexA1-87-pa3899105-170 | This study |
| pSM67 | pDP804 lexA1-87408-pa24671-90 | This study |
| pSM70 | pDP804 lexA1-87408-pa39001-85 | This study |