TABLE 2.
Plasmid | Protein | Region deleted in FecI | β-Galactosidase activity (Miller units)a
|
|
---|---|---|---|---|
FecR1-85 | Wild-type FecR | |||
None | No FecI | 241 | 216 | |
pSM173 | Wild-type FecI | 25 | 36 | |
pSM20 | FecI Δ9-32 | 2.1 | 29 | 27 |
pSM21 | FecI Δ32-55 | 2.2 | 28 | 29 |
pSM22 | FecI Δ52-67 | 2.3 | 23 | 28 |
pSM23 | FecI Δ65-83 | 2.4 | 25 | 32 |
pSM24 | FecI Δ79-114 | 3 | 29 | 71 |
pSM25 | FecI Δ114-134 | 4.1 | 225 | 209 |
pSM26 | FecI Δ133-173 | 4.2 | 213 | 216 |
pSM61 | FecI109-173 | 1-3 | 32 | 31 |
Determined using the bacterial two-hybrid LexA-based system in E. coli SU202 sulA-lacZ.