TABLE 2.
Interaction of FecI deletion derivatives with complete FecR and FecR1-85
| Plasmid | Protein | Region deleted in FecI | β-Galactosidase activity (Miller units)a
|
|
|---|---|---|---|---|
| FecR1-85 | Wild-type FecR | |||
| None | No FecI | 241 | 216 | |
| pSM173 | Wild-type FecI | 25 | 36 | |
| pSM20 | FecI Δ9-32 | 2.1 | 29 | 27 |
| pSM21 | FecI Δ32-55 | 2.2 | 28 | 29 |
| pSM22 | FecI Δ52-67 | 2.3 | 23 | 28 |
| pSM23 | FecI Δ65-83 | 2.4 | 25 | 32 |
| pSM24 | FecI Δ79-114 | 3 | 29 | 71 |
| pSM25 | FecI Δ114-134 | 4.1 | 225 | 209 |
| pSM26 | FecI Δ133-173 | 4.2 | 213 | 216 |
| pSM61 | FecI109-173 | 1-3 | 32 | 31 |
a
Determined using the bacterial two-hybrid LexA-based system in E. coli SU202 sulA-lacZ.