TABLE 3.

Binding of mutated FecI_64-173 to wild-type FecR_1-85

Plasmid	fecI mutation(s)	Amino acid substitution(s) in FecI	β-Galactosidase activity (Miller units)a
pSM76	None	None	25
pSM45	T1570C	L113P	58
pSM49	T1642C	L137P	72
pSM46	T1669C	L146P	180
pSM52	A1613G, T1669G, T1702C	E127E, L146R, V161A	71
pSM47	T1729C	L166P	178
pSM51	T1729C, T1749G	L166P, L173V	205

^aDetermined using the bacterial two-hybrid LexA-based system in E. coli SU202 sulA-lacZ.