TABLE 5.
Interaction of the complete and truncated FecI homologs PA2468 and PA3988 of P. aeruginosa with the truncated FecR homologs PA2467 and PA3900 of P. aeruginosa and FecR1-85 of Escherichia coli
| Plasmids | Protein combination | β-galactosidase activity (Miller units)a |
|---|---|---|
| pMS604, pDP804 | Fos and Junb | 25 |
| pSM65, pSM67 | PA2468 and PA24671-90 | 28 |
| pSM66, pSM67 | PA2468110-172 and PA24671-90 | 14 |
| pSM68, pSM70 | PA3899 and PA39001-85 | 39 |
| pSM69, pSM70 | PA3899105-170 and PA39001-85 | 23 |
| pSM65, pSM70 | PA2468 and PA39001-85 | 397 |
| pSM68, pSM67 | PA3899 and PA24671-90 | 269 |
| pSM65, pSM85 | PA2468 and FecR1-85 | 181 |
a
Determined using the bacterial two-hybrid LexA-based system in E. coli SU202 sulA-lacZ.
b
Dimerization of Jun and Fos was used to develop the LexA-based dimerization assay (33).