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. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: Oncogene. 2005 Mar 24;24(13):2155–2165. doi: 10.1038/sj.onc.1208414

Fig. 8. Interaction of constitutively active α13 mutants with GST-TPR.

Fig. 8

COS-7 cells were transfected with the indicated α13QL constructs (4.5 μg) and pcDNA3 (4.5 μg). α13QL or mutants were pulled down with 10 μg of GST-TPR immobilized on glutathione agarose beads. α13QL or mutant pulldowns were detected by immunoblotting with anti-HA antibody (upper panel). Cell lysates were immunoblotted with an anti-HA antibody to determine equal expression of α13QL mutants (lower panel). Results shown are representative of at least three independent experiments.