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. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: J Cell Sci. 2005 Mar 1;118(Pt 5):863–872. doi: 10.1242/jcs.01658

Fig. 4.

Fig. 4

Onset of optic nerve regenerative failure in P5 Bcl-2tg mice coincides with astrocyte maturation. (A,B) Quantification of retinal axon regrowth in retina-midbrain slice co-cultures. Values are mean±s.e.m. (**P<0.01; ***P<0.001). (C) Photomicrograph montages of adjacent longitudinal optic nerve sections at day 4 after optic nerve crush in a P5 wt mouse and a Bcl-2tg mouse. The asterisk indicates the crush site. Bar, 250 μm. (D,E) Western blot analysis (D) and RT-PCR (E) reveal developmental expression patterns of myelin/oligodendrocyte-associated proteins and astrocyte markers in E14-P14 mouse midbrains. (F) Western blot analysis of GFAP expression in P0 and P5 normal (−) and injured (+) midbrain tissues. (G) Western blot analysis confirms the absence of MBP and MAG in the midbrains of jimpy mice. (H) Quantification of retinal axon regrowth into midbrain slices of P14 wt and jimpy mice in retina-midbrain slice co-cultures. Values are mean±s.e.m. (P=0.6).