Table 1.
Heterogeneity, Relative Abundances, and Extent of Sialylation Determined for Complex Glycans Using nHPLC/ESI/MS
| Glycopeptide Mass | |||||
|---|---|---|---|---|---|
| Site | Tryptic Peptide | aTheo. | bObs. | c(GlcNAc-Gal)x(SA)y +/− Fuc | Relative Abundance(%) |
| N99 | T6 d(K)LTPLBVTLNBTDLGK(A) | 3110.3 | 3110.4 | (GlcNAc-Gal)1 + Fuc | 100 |
| 3401.4 | 3401.8 | (GlcNAc-Gal)1(SA)1 + Fuc | 68 | ||
| 3475.5 | 3475.2 | (GlcNAc-Gal)2 + Fuc | 84 | ||
| 3766.6 | 3767.5 | (GlcNAc-Gal)2(SA)1 + Fuc | 53 | ||
| 4057.7 | 4058.2 | (GlcNAc-Gal)2(SA)2 + Fuc | 53 | ||
| 3840.6 | 3841.5 | (GlcNAc-Gal)3 + Fuc | 74 | ||
| N110 | T7 (K)ATNTNSSNWK(E) | 2745.1 | 2746.1 | (GlcNAc-Gal)2 − Fuc | 79 |
| 3036.2 | 3036.3 | (GlcNAc-Gal)2(SA)1 − Fuc | 42 | ||
| 3327.3 | 3327.1 | (GlcNAc-Gal)2(SA)2 − Fuc | 53 | ||
| 3110.3 | 3110.5 | (GlcNAc-Gal)3 − Fuc | 100 | ||
| 3401.4 | 3400.1 | (GlcNAc-Gal)3(SA)1 − Fuc | 84 | ||
| 3475.5 | 3475.2 | (GlcNAc-Gal)4 − Fuc | 65 | ||
| 3766.6 | 3767.5 | (GlcNAc-Gal)4(SA)1 − Fuc | 53 | ||
| 4057.7 | 4058.7 | (GlcNAc-Gal)4(SA)2 − Fuc | 53 | ||
| N170 | T15 (R)LIHBNR(S) | 2582.0 | 2581.2 | (GlcNAc-Gal)2 + Fuc | 48 |
| 2873.1 | 2872.8 | (GlcNAc-Gal)2(SA)1 + Fuc | 98 | ||
| 3164.2 | 3164.5 | (GlcNAc-Gal)2(SA)2 + Fuc | 100 | ||
| 2947.2 | 2945.7 | (GlcNAc-Gal)3 + Fuc | 35 | ||
| 3238.3 | 3237.2 | (GlcNAc-Gal)3(SA)1 + Fuc | 39 | ||
| 3529.4 | 3528.9 | (GlcNAc-Gal)3(SA)2 + Fuc | 55 | ||
| 3820.5 | 3819.9 | (GlcNAc-Gal)3(SA)3+ Fuc | 29 | ||
| 3312.3 | 3310.2 | (GlcNAc-Gal)4 + Fuc | 19 | ||
| 3603.4 | 3602.8 | (GlcNAc-Gal)4(SA)1 + Fuc | 29 | ||
| 3894.5 | 3893.6 | (GlcNAc-Gal)4(SA)2 + Fuc | 35 | ||
| 4185.6 | 4186.1 | (GlcNAc-Gal)4(SA)3 + Fuc | 24 | ||
| N249 | T21 (R)SDNFTNNAK(T) | 2779.1 | 2777.9 | (GlcNAc-Gal)2 + Fuc | 68 |
| 3070.2 | 3069.8 | (GlcNAc-Gal)2(SA)1 + Fuc | 100 | ||
| 3361.3 | 3362.8 | (GlcNAc-Gal)2(SA)2 + Fuc | 45 | ||
| 3144.2 | 3142.7 | (GlcNAc-Gal)3 + Fuc | 50 | ||
| 3435.3 | 3434.2 | (GlcNAc-Gal)3(SA)1 + Fuc | 64 | ||
| 2998.2 | 2997.5 | (GlcNAc-Gal)3 − Fuc | 73 | ||
| 3289.3 | 3288.2 | (GlcNAc-Gal)3(SA)1 − Fuc | 50 | ||
| 3580.4 | 3579.7 | (GlcNAc-Gal)3(SA)2 − Fuc | 64 | ||
| 3871.5 | 3872.3 | (GlcNAc-Gal)3(SA)3 − Fuc | 55 | ||
| N274 | T22 (K)TIIVQLNESVAINBTRPNNNTR(K) | 3932.3 | 3930.9 | (GlcNAc-Gal)1 + Fuc | 12 |
| 4223.4 | 4222.2 | (GlcNAc-Gal)1(SA)1 + Fuc | 16 | ||
| 4297.5 | 4296.7 | (GlcNAc-Gal)2 + Fuc | 88 | ||
| 4588.6 | 4587.5 | (GlcNAc-Gal)2(SA)1 + Fuc | 78 | ||
| 4662.6 | 4661.6 | (GlcNAc-Gal)3 + Fuc | 39 | ||
| 4953.7 | 4954.1 | (GlcNAc-Gal)3(SA)1 + Fuc | 37 | ||
| 5244.8 | 5243.8 | (GlcNAc-Gal)3(SA)2 + Fuc | 27 | ||
| 5027.7 | 5028.8 | (GlcNAc-Gal)4 + Fuc | 22 | ||
| 5318.8 | 5319.9 | (GlcNAc-Gal)4(SA)1 + Fuc | 100 | ||
| 5609.9 | 5609.5 | (GlcNAc-Gal)4(SA)2 + Fuc | 63 | ||
| N415 | T39 (K)AMYAPPIGGQISBSSNITGLLLTR(D) | 3925.5 | 3926.1 | (GlcNAc-Gal)1 + Fuc | 14 |
| 4216.5 | 4216.1 | (GlcNAc-Gal)1(SA)1 + Fuc | 13 | ||
| 4290.6 | 4291.1 | (GlcNAc-Gal)2 + Fuc | 100 | ||
| 4581.7 | 4582.4 | (GlcNAc-Gal)2(SA)1 + Fuc | 69 | ||
| 4655.7 | 4656.2 | (GlcNAc-Gal)3 + Fuc | 20 | ||
| 4946.8 | 4947.2 | (GlcNAc-Gal)3(SA)1 + Fuc | 42 | ||
| 5237.9 | 5237.6 | (GlcNAc-Gal)3(SA)2 + Fuc | 31 | ||
| N428e(P)/N431 | T40 (R)DGGTNVTNDTEVFRPGGGDMR(D) | 3598.5 | 3597.9 | (GlcNAc-Gal)1 + Fuc | 4 |
| 3889.6 | 3888.6 | (GlcNAc-Gal)1(SA)1 + Fuc | 6 | ||
| 3963.6 | 3963.5 | (GlcNAc-Gal)2 + Fuc | 5 | ||
| 4254.7 | 4254.3 | (GlcNAc-Gal)2(SA)1 + Fuc | 100 | ||
| 4545.8 | 4544.9 | (GlcNAc-Gal)2(SA)2 + Fuc | 90 | ||
| 4328.8 | 4328.2 | (GlcNAc-Gal)3 + Fuc | 2 | ||
| 4619.9 | 4618.8 | (GlcNAc-Gal)3(SA)1 + Fuc | 7 | ||
| 4910.9 | 4911.5 | (GlcNAc-Gal)3(SA)2 + Fuc | 16 | ||
| 5202.0 | 5201.1 | (GlcNAc-Gal)3(SA)3 + Fuc | 9 | ||
a
Theo. = theoretical
b
Obs. = observed
c
GlcNAc-Gal = N-acetylglucosamine and galactose, x = the number of GlcNAc-Gal units, SA = sialic acid, y = the number of SA units, and Fuc = fucose
d
B = carboxymethylcysteine
e
P = partially glycosylated