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. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: Traffic. 2005 Nov;6(11):1036–1046. doi: 10.1111/j.1600-0854.2005.00343.x

Figure 4. Motor binding to microtubules.

Figure 4

A) Microtubules alone. Microtubules negatively stained without motors have a pale outline of negative stain. B) Wild-type Ncd bound to microtubules. Two microtubules are shown after incubation with excess wild-type Ncd motor protein, negative staining and visualization by electron microscopy (EM). One microtubule (top) appears fully decorated with motors, as indicated by the continuous dark outline; the other (bottom) with a paler outline appears almost bare of motors. C) NcdNK11 bound to microtubules. Microtubules incubated with the NcdNK11 mutant motor are fully decorated (top) or almost bare (bottom), as observed for wild-type Ncd. Bar, 0.2 μm.