Table 2.
Effects of NcdNK11 on mitotic spindles. Females were homozygous for cand and the wild-type ncdgfp* or ncdnk11gfp* transgene. Time is microtubule nucleation in prometaphase to midbody formation in telophase; s, seconds. M to A, spindle length increase from metaphase to late anaphase, just prior to midbody formation. Values = mean ± SEM
| Pole-to-pole spindle length (μm, n = 47) |
|||||
|---|---|---|---|---|---|
| Time (s) | Interphase | Metaphase | Anaphase | M to A | |
| Wild-type ncdgfp* #4121 | 253 ± 7 (n = 17) | 9.5 ± 0.2 | 12.3 ± 0.1 | 15.7 ± 0.1 | 3.4 ± 0.1 |
| ncdnk11gfp* M25M2 | 263 ± 7 (n = 14) | 10.1 ± 0.2 | 12.6 ± 0.2 | 16.7 ± 0.2 | 4.1 ± 0.1 |
| ncdnk11gfp* M25M3#10 | 270 ± 8 (n = 16) | 10.3 ± 0.1 | 13.1 ± 0.1 | 17.9 ± 0.2 | 4.8 ± 0.1 |