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. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: J Biol Chem. 2003 Dec 18;279(11):10422–10432. doi: 10.1074/jbc.M312410200

Fig. 4. Chromatographic separation of nonpolar retinoids from Lrat mouse eyes.

Fig. 4

Retinoids were extracted from the eye and separated on normal phase HPLC as described under “Materials and Methods.” The peaks correspond to the following retinoids: 1, 13-cis-retinyl esters/11-cis-retinyl esters; 2, all-trans-retinyl esters; 3, 3′, syn-and anti-11-cis-retinal oximes; 4, 4′, syn- and anti-all-trans-retinal oximes; 5, 11-cis-retinol; 6, all-trans-retinol. * indicates artifacts related to a change in the solvent composition. Pure hexane was used as a control. Note that retinals are detected after conversion to oximes with NH2OH·HCl.