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. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: Biochem Pharmacol. 2003 Nov 1;66(9):1755–1768. doi: 10.1016/s0006-2952(03)00542-2

FIGURE 1.

FIGURE 1

A. K562/HS2γ luciferase screening construct. LCR hypersensitive site 2 (HS2) was linked to the γ-globin proximal promoter and inserted 5′ to a luciferase reporter gene from P. pyralis. TK-neo was added and the resulting plasmid DNA was linearized and stably integrated in K562 cells by electroporation. B. K562/γYAC luciferase screening construct. A luciferase reporter gene was recombined into the Aγ-globin gene within a 540kb yeast artificial chromosome (YAC; Groveld et al., 1996). Recombination was performed such that the ATG of luciferase displaced the ATG of Aγ. The resulting luciferase’tagged’ γ-globin gene was stably integrated in K562 cells by spheroplast fusion.