Table 1.

Divalent cation-dependence for binding of ¹²⁵I-thrombospondin-1 to immobilized TSG-6Q and Link_TSG6. Apparent dissociation constants ± SD for the specified buffers and temperatures were determined from self displacement data by nonlinear regression using the LIGAND programs.

	Kd (M)
	PBS Ca2+/Mg2+ 37° C	PBS/EDTA 37° C	PBS Ca2+/Mg2+ 22° C
Link_TSG6	8.0 ± 3.1 x 10−8	3.0 ± 1.2 x 10−8	1.13±0.01x10−7
Full-length TSG-6Q	3.1 ± 0.6 x 10−7	~2.4 x 10−6	1.2±0.1x10−7