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. Author manuscript; available in PMC: 2006 Jan 25.
Published in final edited form as: J Biol Chem. 1995 Jun 23;270(25):14983–14988. doi: 10.1074/jbc.270.25.14983

Fig. 2.

Fig. 2

Complementation of the CTP synthetase defect of the S. cerevisiae ura7Δ ura8Δ mutant by the human CTPS1 or CTPS2 gene. Strain SD195, a ura7Δ ura8Δ mutant carrying the URA7 gene on a URA3-based plasmid (pDO134), was transformed to leucine prototrophy with the empty LEU2-based plasmid (pDO105), or with the same plasmid containing the human CTP synthetase genes (pDO105-hCTPS1 or pDO105-hCTPS2). The transformants were streaked onto SC-leucine plates containing 5FOA and incubated for 3 days at 30 °C. The 5FOA-resistant colonies were produced only from the transformants carrying pDO105-hCTPS1 or pDO105-hCTPS2. Four independent colonies from 5FOA-resistant cells were patched onto SC-leucine plates and grown for 2 days at 30 °C. Strain SD195 carrying pDO105 was included in this analysis as a negative control. The patches were replica plated onto SC-leucine, SC-leucine + 5FOA, and SC-uracil plates. The plates were incubated for 3 days at 30 °C.